Hotspot

rps8-rpl14

Genome · 75853-76570 · 718 bp

Back to Tibetia

Pi 0.00557

MarkerSeek score 53.00

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: Genome
length_bp: 718
variable_sites: 6
pi: 0.00557
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ACTCATGTCAGCATTGCGTA	AATAGCCCGCGAATTGAGAC	45.0	50.0	57.6	58.4	838-842 bp	1.0	76.1
rps8-rpl14_p2	TACTCATGTCAGCATTGCGT	AATAGCCCGCGAATTGAGAC	45.0	50.0	57.6	58.4	839-843 bp	1.0	76.1
rps8-rpl14_p3	ACTCATGTCAGCATTGCGTA	CAATAGCCCGCGAATTGAGA	45.0	50.0	57.6	58.4	839-843 bp	1.0	76.0
rps8-rpl14_p4	TACTCATGTCAGCATTGCGT	CAATAGCCCGCGAATTGAGA	45.0	50.0	57.6	58.4	840-844 bp	1.0	76.0
rps8-rpl14_p5	TCAGCATTGCGTATCAAGGT	AATAGCCCGCGAATTGAGAC	45.0	50.0	57.6	58.4	831-835 bp	1.0	75.9