Hotspot

psbT-psbN

Genome · 69687-69751 · 65 bp

Back to Tibetia

Pi 0.0615

MarkerSeek score 75.80

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: Genome
length_bp: 65
variable_sites: 6
pi: 0.0615
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	AGAACCCCCTATAGTTCCAACT	CCATTCGAAGAACATGGGGA	45.5	50.0	57.9	57.6	126 bp	1.0	64.4
psbT-psbN_p2	AGAGAACCCCCTATAGTTCCA	CCATTCGAAGAACATGGGGA	47.6	50.0	56.9	57.6	128 bp	1.0	64.3
psbT-psbN_p3	AGAGAACCCCCTATAGTTCCA	TCCATTCGAAGAACATGGGG	47.6	50.0	56.9	57.6	129 bp	1.0	64.3
psbT-psbN_p4	AGAACCCCCTATAGTTCCAACT	TCCATTCGAAGAACATGGGG	45.5	50.0	57.9	57.6	127 bp	1.0	64.3
psbT-psbN_p5	AGAGAACCCCCTATAGTTCCA	GGCAACCCTCTCAACAACTA	47.6	50.0	56.9	57.4	154 bp	1.0	62.5