Hotspot

clpP-psbB

Genome · 67394-67856 · 463 bp

Back to Tibetia

Pi 0.0389

MarkerSeek score 65.60

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: Genome
length_bp: 463
variable_sites: 27
pi: 0.0389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CTACCCGAGGAATACCGACT	TGGACAGAAAGTAAACGGCC	55.0	50.0	58.0	58.1	556-1114 bp	1.0	85.8
clpP-psbB_p2	CTACCCGAGGAATACCGACT	AAACGGCCGGGATCATTTAA	55.0	45.0	58.0	57.9	544-1102 bp	1.0	85.7
clpP-psbB_p3	ATGCATCCTCATCTCCAGGA	TGGACAGAAAGTAAACGGCC	50.0	50.0	57.9	58.1	586-1144 bp	1.0	84.8
clpP-psbB_p4	TGCATCCTCATCTCCAGGAA	TGGACAGAAAGTAAACGGCC	50.0	50.0	58.1	58.1	585-1143 bp	1.0	84.8
clpP-psbB_p5	ATGCATCCTCATCTCCAGGA	AAACGGCCGGGATCATTTAA	50.0	45.0	57.9	57.9	574-1132 bp	1.0	84.8