Hotspot

trnP-UGG-psaJ

Genome · 63093-63608 · 516 bp

Back to Tibetia

Pi 0.00646

MarkerSeek score 54.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 516
variable_sites: 5
pi: 0.00646
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	CGGTGCCACAGAGAGATATG	50.0	55.0	58.1	57.9	607-640 bp	1.0	81.9
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	CGGTGCCACAGAGAGATATG	50.0	55.0	58.1	57.9	606-639 bp	1.0	81.9
trnP-UGG-psaJ_p3	ACCCGTGACATTTTGTACCC	CGAAGCATAGAGTACTGGCC	50.0	55.0	58.1	57.9	629-662 bp	1.0	81.8
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	CGAAGCATAGAGTACTGGCC	50.0	55.0	58.1	57.9	628-661 bp	1.0	81.8
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	CGAAGCATAGAGTACTGGCC	50.0	55.0	58.1	57.9	640-673 bp	1.0	81.7