Hotspot

psbK-trnQ-UUG

Genome · 53299-53954 · 656 bp

Back to Tibetia

Pi 0.00915
MarkerSeek score 51.10
Primer available yes
Label psbK-trnQ-UUG

Feature Metadata

label_name
psbK-trnQ-UUG
feature_type
igs
region
Genome
length_bp
656
variable_sites
9
pi
0.00915
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-trnQ-UUG_p1 AGCTTCAGGTAATTTCGCCA GACCAAAACCCGTTGCCTTA 45.0 50.0 57.5 58.7 781-794 bp 1.0 71.7
psbK-trnQ-UUG_p2 AGCTTCAGGTAATTTCGCCA CGAATAACGGGACCAAAACC 45.0 50.0 57.5 57.1 791-804 bp 1.0 69.8
psbK-trnQ-UUG_p3 AGCTTCAGGTAATTTCGCCA ACCAAAACCCGTTGCCTTAC 45.0 50.0 57.5 59.0 780-793 bp 1.0 69.4
psbK-trnQ-UUG_p4 AGCTTCAGGTAATTTCGCCA GAATAACGGGACCAAAACCC 45.0 50.0 57.5 56.7 790-803 bp 1.0 66.6
psbK-trnQ-UUG_p5 GCTTCAGGTAATTTCGCCAAG GACCAAAACCCGTTGCCTTA 47.6 50.0 57.9 58.7 780-793 bp 1.0 66.5