Hotspot

trnG-UCC-trnS-GCU

Genome · 51558-52319 · 762 bp

Back to Tibetia

Pi 0.0245

MarkerSeek score 67.50

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: Genome
length_bp: 762
variable_sites: 28
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	AGGGTCCCTAGAACGAATCA	CAATCCGACGCTTTAGTCCA	50.0	50.0	57.5	58.0	831-843 bp	1.0	82.3
trnG-UCC-trnS-GCU_p2	AGGGTCCCTAGAACGAATCA	AATCCGACGCTTTAGTCCAC	50.0	50.0	57.5	58.0	830-842 bp	1.0	82.3
trnG-UCC-trnS-GCU_p3	GGGTCCCTAGAACGAATCAC	CAATCCGACGCTTTAGTCCA	55.0	50.0	57.4	58.0	830-842 bp	1.0	81.8
trnG-UCC-trnS-GCU_p4	GGGTCCCTAGAACGAATCAC	AATCCGACGCTTTAGTCCAC	55.0	50.0	57.4	58.0	829-841 bp	1.0	81.8
trnG-UCC-trnS-GCU_p5	AGGGTCCCTAGAACGAATCA	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.5	58.2	884-896 bp	1.0	80.8