Hotspot

trnS-UGA-psbC

Genome · 25508-25705 · 198 bp

Back to Tibetia

Pi 0.0269

MarkerSeek score 57.80

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 198
variable_sites: 8
pi: 0.0269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	GAGAGATGGCTGAGTGGTTG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.0	58.4	396-397 bp	1.0	78.3
trnS-UGA-psbC_p2	GCTGAGTGGTTGATAGCTCC	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	388-389 bp	1.0	78.0
trnS-UGA-psbC_p3	GGCTGAGTGGTTGATAGCTC	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	389-390 bp	1.0	78.0
trnS-UGA-psbC_p4	GGTTGATAGCTCCGGTCTTG	TAGGTCATTTATGGCACGCG	55.0	50.0	58.1	58.4	381-382 bp	1.0	78.0
trnS-UGA-psbC_p5	CTGAGTGGTTGATAGCTCCG	TAGGTCATTTATGGCACGCG	55.0	50.0	57.8	58.4	387-388 bp	1.0	76.7