Hotspot

rps15

SSC · 124694-124966 · 273 bp

Back to Tiarella

Pi 0.00733

MarkerSeek score 46.10

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 3
pi: 0.00733
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	GAAAGGAGTCTGTGCACCAA	GAGTGCCGTCCCTTTGTATT	50.0	50.0	58.0	57.9	973-980 bp	1.0	80.1
rps15_p2	TCTGTGCACCAATATCTGCC	GAGTGCCGTCCCTTTGTATT	50.0	50.0	58.0	57.9	965-972 bp	1.0	79.9
rps15_p3	GAAAGGAGTCTGTGCACCAA	TTATGAGTGCCGTCCCTTTG	50.0	50.0	58.0	57.9	984 bp	0.666667	65.9
rps15_p4	TCTGTGCACCAATATCTGCC	TTATGAGTGCCGTCCCTTTG	50.0	50.0	58.0	57.9	976 bp	0.666667	65.8
rps15_p5	AACACCATGCATTGATGGGT	TTATGAGTGCCGTCCCTTTG	45.0	50.0	58.1	57.9	666 bp	0.666667	65.8