Hotspot

rpl32-trnL-UAG

SSC · 115197-115678 · 482 bp

Back to Tiarella

Pi 0.0207

MarkerSeek score 68.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 482
variable_sites: 15
pi: 0.0207
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCGTTAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	602-603 bp	1.0	81.9
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCGTTAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	603-604 bp	1.0	81.9
rpl32-trnL-UAG_p3	GGGATATTGGGCAGCGTTAA	TTTAGAAGATCCCATGCCGC	50.0	50.0	58.0	58.0	667-668 bp	1.0	81.5
rpl32-trnL-UAG_p4	GGGATATTGGGCAGCGTTAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	612-613 bp	1.0	80.9
rpl32-trnL-UAG_p5	GGGCAGCGTTAAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	594-595 bp	1.0	80.9