Hotspot

petG-trnW-CCA

LSC · 68158-68262 · 105 bp

Back to Tiarella

Pi 0.0254

MarkerSeek score 68.70

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 105
variable_sites: 4
pi: 0.0254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	187 bp	1.0	80.2
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	CAGTTTGGTAGAACGTGGGT	50.0	50.0	57.8	57.8	196 bp	1.0	78.4
petG-trnW-CCA_p3	CAGACGTGGTGATCAGTTGG	AGAACGTGGGTCTCCAAAAC	55.0	50.0	58.6	58.0	186 bp	1.0	76.9
petG-trnW-CCA_p4	GTGGTGATCAGTTGGACCTT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.4	58.0	181 bp	1.0	76.9
petG-trnW-CCA_p5	GACGTGGTGATCAGTTGGAC	AGAACGTGGGTCTCCAAAAC	55.0	50.0	58.6	58.0	184 bp	1.0	76.9