Hotspot

accD-psaI

LSC · 61155-61792 · 638 bp

Back to Tiarella

Pi 0.00627

MarkerSeek score 62.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 638
variable_sites: 6
pi: 0.00627
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.8	58.0	751-757 bp	1.0	74.3
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.8	57.9	757-763 bp	1.0	73.7
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	52.6	45.0	58.1	58.0	751-757 bp	1.0	71.9
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	42.9	45.0	58.1	58.0	749-755 bp	1.0	71.8
accD-psaI_p5	GAGTTATTTCAGCTCCACGC	CCATTGCAATTGCCGGAAAT	50.0	45.0	56.9	58.0	762-768 bp	1.0	71.6