Hotspot

psbC-trnS-UGA

LSC · 36974-37205 · 232 bp

Back to Tiarella

Pi 0.0263

MarkerSeek score 71.80

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 232
variable_sites: 9
pi: 0.0263
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	CCTGTTCTTTCCATGACCCC	GAGAGATGGCTGAGTGGTTG	55.0	55.0	58.2	58.0	348-354 bp	1.0	82.0
psbC-trnS-UGA_p2	CCTGTTCTTTCCATGACCCC	GCTGAGTGGTTGATAGCTCC	55.0	55.0	58.2	58.1	340-346 bp	1.0	81.8
psbC-trnS-UGA_p3	CCTGTTCTTTCCATGACCCC	GGCTGAGTGGTTGATAGCTC	55.0	55.0	58.2	58.1	341-347 bp	1.0	81.8
psbC-trnS-UGA_p4	CCTGTTCTTTCCATGACCCC	GGTTGATAGCTCCGGTCTTG	55.0	55.0	58.2	58.1	333-339 bp	1.0	81.8
psbC-trnS-UGA_p5	TCGTAGGTCATTTGTGGCAC	GAGAGATGGCTGAGTGGTTG	50.0	55.0	58.2	58.0	428-434 bp	1.0	81.0