Hotspot

rpoC2

LSC · 26838-31355 · 4518 bp

Back to Thyrsostachys

Pi 0.00199

MarkerSeek score 40.40

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4518
variable_sites: 9
pi: 0.00199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	CTGGTCGAATTAGGGGAAGC	TCGATTCAACAAGGTGCCAA	55.0	45.0	58.0	58.0	2211-2253 bp	1.0	79.7
rpoC2_p2	TTCGCTCCACAAGGATCAAG	GGGCTCCCATATTCGTCTTC	50.0	55.0	57.9	57.8	723-765 bp	1.0	78.4
rpoC2_p3	AATCATGTCCAACGGCCATT	GGGCTCCCATATTCGTCTTC	45.0	55.0	58.1	57.8	561-603 bp	1.0	78.1
rpoC2_p4	TTCGCTCCACAAGGATCAAG	ACCAATCCACCTAGTCGACT	50.0	50.0	57.9	57.8	1020-1062 bp	1.0	77.7
rpoC2_p5	TTCGCTCCACAAGGATCAAG	TTAGGGATGAGGATCTGGGC	50.0	55.0	57.9	58.3	944-986 bp	1.0	77.5