Hotspot

ndhF

SSC · 104890-107109 · 2220 bp

Back to Thyrsostachys

Pi 0.0018

MarkerSeek score 45.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 4
pi: 0.0018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGAGACAGGGGAATTCCTT	TGGTTTGGTGAATCGGAAACA	50.0	42.9	57.6	58.3	2312 bp	0.5	46.3
ndhF_p2	CAGGAGACAGGGGAATTCCT	TGGTTTGGTGAATCGGAAACA	55.0	42.9	58.4	58.3	2313 bp	0.5	46.0
ndhF_p3	AGGAGACAGGGGAATTCCTT	GGTTTGGTGAATCGGAAACA	50.0	45.0	57.6	56.5	2311 bp	0.5	45.4
ndhF_p4	AGGAGACAGGGGAATTCCTT	TGGTTTGGTGAATCGGAAAC	50.0	45.0	57.6	56.5	2312 bp	0.5	45.4
ndhF_p5	CAGGAGACAGGGGAATTCCT	GGTTTGGTGAATCGGAAACA	55.0	45.0	58.4	56.5	2312 bp	0.5	45.1