Hotspot

clpP-psbB

LSC · 70639-71150 · 512 bp

Back to Thyrsostachys

Pi 0.00588

MarkerSeek score 60.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 512
variable_sites: 3
pi: 0.00588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	ACCCAAGTCGCTTCTTCATC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.9	58.0	643-644 bp	1.0	79.7
clpP-psbB_p2	TCAACCCAAGTCGCTTCTTC	TATGCACCGAAAGCAATCGA	50.0	45.0	58.1	58.0	646-647 bp	1.0	79.5
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	TATGCACCGAAAGCAATCGA	50.0	45.0	57.8	58.0	603-604 bp	1.0	79.2
clpP-psbB_p4	ACCCAAGTCGCTTCTTCATC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.9	57.9	675-676 bp	1.0	78.9
clpP-psbB_p5	TCAACCCAAGTCGCTTCTTC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.1	57.9	678-679 bp	1.0	78.7