Hotspot

rpl32-trnL-UAG

SSC · 111347-112072 · 726 bp

Back to Thymus

Pi 0.00672
MarkerSeek score 60.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
726
variable_sites
27
pi
0.00672
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGAAAGGTTATTGGGCGG GATGATATGCCATGCCGCTA 50.0 50.0 58.2 58.0 914-945 bp 1.0 84.0
rpl32-trnL-UAG_p2 AAGGAAAGGTTATTGGGCGG TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 861-892 bp 1.0 83.5
rpl32-trnL-UAG_p3 AAGGAAAGGTTATTGGGCGG AGCAGCGTGTCTACCAATTT 50.0 45.0 58.2 57.8 854-885 bp 1.0 83.0
rpl32-trnL-UAG_p4 AAGGAAAGGTTATTGGGCGG CCTAAGAGCAGCGTGTCTAC 50.0 55.0 58.2 57.8 860-891 bp 1.0 82.9
rpl32-trnL-UAG_p5 AAGGAAAGGTTATTGGGCGG CTAAGAGCAGCGTGTCTACC 50.0 55.0 58.2 57.8 859-890 bp 1.0 82.9