Hotspot

infA-rps8

LSC · 78863-78989 · 127 bp

Back to Thymus

Pi 0.0214
MarkerSeek score 67.10
Primer available yes
Label infA-rps8

Feature Metadata

label_name
infA-rps8
feature_type
igs
region
LSC
length_bp
127
variable_sites
9
pi
0.0214
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
infA-rps8_p1 CGTTGGGAAGCGATTCAGTA GAAGGAATCGGCGGAGAAAT 50.0 50.0 58.0 58.0 213-222 bp 1.0 86.9
infA-rps8_p2 CGTTGGGAAGCGATTCAGTA ATCGGGAAGCTCGACTAGAA 50.0 50.0 58.0 57.9 230-239 bp 1.0 86.3
infA-rps8_p3 CGTTGGGAAGCGATTCAGTA TAGAAGGAATCGGCGGAGAA 50.0 50.0 58.0 58.2 215-224 bp 1.0 85.4
infA-rps8_p4 CGTTGGGAAGCGATTCAGTA GGGAAGCTCGACTAGAAGGA 50.0 55.0 58.0 58.2 227-236 bp 1.0 85.0
infA-rps8_p5 CGTTGGGAAGCGATTCAGTA AGATCGGGAAGCTCGACTAG 50.0 55.0 58.0 58.4 232-241 bp 1.0 83.5