Hotspot

trnT-UGU-trnL-UAA

LSC · 46087-46762 · 676 bp

Back to Thymus

Pi 0.00881
MarkerSeek score 55.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
676
variable_sites
33
pi
0.00881
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 710-745 bp 1.0 84.5
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 742-777 bp 1.0 83.5
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 704-739 bp 1.0 83.3
trnT-UGU-trnL-UAA_p4 GGAATCGAACCGATGACCAT CGTCTACCGATTTCGCCATA 50.0 50.0 57.8 57.6 736-771 bp 1.0 82.3
trnT-UGU-trnL-UAA_p5 CTAACCTCTGAGCTAAGCGG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.5 58.2 702-737 bp 1.0 81.6