Hotspot

psaA-ycf3

LSC · 41106-41870 · 765 bp

Pi 0.00547

MarkerSeek score 58.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 22
pi: 0.00547
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	877-882 bp	1.0	81.6
psaA-ycf3_p2	GAAAAATGACCCGGTTTGGC	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.2	57.9	895-900 bp	1.0	80.8
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	950-955 bp	1.0	80.2
psaA-ycf3_p4	GAAAAATGACCCGGTTTGGC	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.2	57.7	968-973 bp	1.0	79.3
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CGGAGGCTTGGTTTGATCAA	50.0	50.0	57.9	58.5	958-963 bp	1.0	78.7