Hotspot

petN-psbM

LSC · 28176-29151 · 976 bp

Back to Thymus

Pi 0.00537
MarkerSeek score 59.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
976
variable_sites
26
pi
0.00537
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT AGTTCCTACCGCTTTTCTACT 50.0 42.9 58.2 56.4 1105-1126 bp 1.0 61.6
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TCATTCTAGTTCCTACCGCT 50.0 45.0 58.2 55.4 1112-1133 bp 1.0 61.4
petN-psbM_p3 TAGTAAGTCTCGCTTGGGCT TCATTCTAGTTCCTACCGCTT 50.0 42.9 58.2 56.2 1112-1133 bp 1.0 59.8
petN-psbM_p4 TAGTAAGTCTCGCTTGGGCT TTCATTCTAGTTCCTACCGCT 50.0 42.9 58.2 56.2 1113-1134 bp 1.0 59.8
petN-psbM_p5 TAGTAAGTCTCGCTTGGGCT AGTTCCTACCGCTTTTCTACTT 50.0 40.9 58.2 57.1 1105-1126 bp 1.0 59.3