Hotspot

rpoB-trnC-GCA

LSC · 26726-27854 · 1129 bp

Back to Thymus

Pi 0.00579
MarkerSeek score 57.60
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1129
variable_sites
33
pi
0.00579
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AGTTCTTCTGCCAAACCCTG TTTTGGTGATTCAGGCGACA 50.0 45.0 58.0 58.0 1304-1327 bp 1.0 82.9
rpoB-trnC-GCA_p2 GTTCTTCTGCCAAACCCTGA TTTTGGTGATTCAGGCGACA 50.0 45.0 58.0 58.0 1303-1326 bp 1.0 82.9
rpoB-trnC-GCA_p3 AGTTCTTCTGCCAAACCCTG CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1283-1306 bp 1.0 82.5
rpoB-trnC-GCA_p4 GTTCTTCTGCCAAACCCTGA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.0 58.1 1282-1305 bp 1.0 82.5
rpoB-trnC-GCA_p5 GCCAAACCCTGATCGATGAA TTTTGGTGATTCAGGCGACA 50.0 45.0 58.2 58.0 1295-1318 bp 1.0 81.0