Hotspot

rpoC2-rpoC1

LSC · 20533-20681 · 149 bp

Back to Thymus

Pi 0.0521
MarkerSeek score 69.00
Primer available yes
Label rpoC2-rpoC1

Feature Metadata

label_name
rpoC2-rpoC1
feature_type
igs
region
LSC
length_bp
149
variable_sites
43
pi
0.0521
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC2-rpoC1_p1 GCCATTCCGAAGTGCTCTAT GGCCTGTTCATATGGTATCT 50.0 45.0 57.8 54.3 281-292 bp 0.916667 47.7
rpoC2-rpoC1_p2 TGAAAGACCAGATTGGCTCG GGCCTGTTCATATGGTATCT 50.0 45.0 57.9 54.3 212 bp 0.833333 44.6
rpoC2-rpoC1_p3 AAGACCAGATTGGCTCGTTC GGCCTGTTCATATGGTATCT 50.0 45.0 57.9 54.3 209 bp 0.833333 44.6
rpoC2-rpoC1_p4 GAAAGACCAGATTGGCTCGT GGCCTGTTCATATGGTATCT 50.0 45.0 57.9 54.3 211 bp 0.833333 44.6
rpoC2-rpoC1_p5 TATGCCATTCCGAAGTGCTC GGCCTGTTCATATGGTATCT 50.0 45.0 58.0 54.3 284 bp 0.833333 44.5