Hotspot

rps16-trnQ-UUG

LSC · 5918-6832 · 915 bp

Back to Thymus

Pi 0.00881
MarkerSeek score 53.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
915
variable_sites
38
pi
0.00881
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 983-1035 bp 1.0 83.8
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GTCTCGCTATTCGGAGGTTC 45.0 55.0 58.1 57.9 996-1048 bp 1.0 83.3
rps16-trnQ-UUG_p3 AAAAATCCACAACGGGTCGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.3 58.1 1010-1062 bp 1.0 82.3
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CGGGTTTTGGTCTCGCTATT 45.0 50.0 58.1 58.3 1005-1057 bp 1.0 82.1
rps16-trnQ-UUG_p5 AAAAATCCACAACGGGTCGT GTCTCGCTATTCGGAGGTTC 45.0 55.0 58.3 57.9 1023-1075 bp 1.0 81.8