Hotspot

rpl36-rps8

LSC · 81119-81636 · 518 bp

Back to Thymelaea

Pi 0.0465

MarkerSeek score 71.70

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 518
variable_sites: 24
pi: 0.0465
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	AATAATTCGTCCCCTCCTGC	TCGGCGGAGAAATTTTGTGT	50.0	45.0	57.4	58.4	622-631 bp	1.0	75.0
rpl36-rps8_p2	AATAATTCGTCCCCTCCTGC	CGGCGGAGAAATTTTGTGTT	50.0	45.0	57.4	57.6	621-630 bp	1.0	74.9
rpl36-rps8_p3	AATAATTCGTCCCCTCCTGC	ATCGGCGGAGAAATTTTGTG	50.0	45.0	57.4	57.1	623-632 bp	1.0	70.8
rpl36-rps8_p4	AATAATTCGTCCCCTCCTGC	AATCGGCGGAGAAATTTTGTG	50.0	42.9	57.4	57.8	624-633 bp	1.0	68.5
rpl36-rps8_p5	ACAATAATTCGTCCCCTCCTG	TCGGCGGAGAAATTTTGTGT	47.6	45.0	57.4	58.4	624-633 bp	1.0	67.6