Hotspot

psbE-petL

LSC · 66928-68355 · 1428 bp

Back to Thymelaea

Pi 0.0284

MarkerSeek score 72.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1428
variable_sites: 39
pi: 0.0284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCTGAGGTTATAGTTAAAGCCA	50.0	39.1	57.9	57.3	1559-1592 bp	1.0	54.7
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGTCGTATCTTACTCAGACCA	50.0	42.9	57.9	55.3	1588-1621 bp	1.0	54.5
psbE-petL_p3	CAACCCGCAATGAATAGGGA	GCTGAGGTTATAGTTAAAGCCAC	50.0	43.5	57.9	57.3	1558-1591 bp	1.0	54.4
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	AGCTGAGGTTATAGTTAAAGCCA	50.0	39.1	57.8	57.3	1482-1515 bp	1.0	54.1
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCTGAGGTTATAGTTAAAGCCA	45.0	39.1	58.2	57.3	1568-1601 bp	1.0	53.7