Hotspot

psbF-psbE

LSC · 66667-66675 · 9 bp

Back to Thymelaea

Pi 0.111

MarkerSeek score 76.90

Primer available yes

Label psbF-psbE

Feature Metadata

label_name: psbF-psbE
feature_type: igs
region: LSC
length_bp: 9
variable_sites: 1
pi: 0.111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbF-psbE_p1	GCCAACCATCGCACTGTAAA	AACCGGTCGTTTTGATCCTT	50.0	45.0	59.1	57.7	111 bp	1.0	69.6
psbF-psbE_p2	GCCAACCATCGCACTGTAAA	ACCGGTCGTTTTGATCCTTT	50.0	45.0	59.1	57.7	110 bp	1.0	69.6
psbF-psbE_p3	CCAACCATCGCACTGTAAAA	AACCGGTCGTTTTGATCCTT	45.0	45.0	56.6	57.7	110 bp	1.0	67.7
psbF-psbE_p4	CCAACCATCGCACTGTAAAA	ACCGGTCGTTTTGATCCTTT	45.0	45.0	56.6	57.7	109 bp	1.0	67.7
psbF-psbE_p5	GCCAACCATCGCACTGTAA	ACCGGTCGTTTTGATCCTTT	52.6	45.0	58.4	57.7	110 bp	1.0	67.0