Hotspot

ndhC-trnV-UAC

LSC · 53131-53724 · 594 bp

Back to Thymelaea

Pi 0.0394
MarkerSeek score 72.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
594
variable_sites
23
pi
0.0394
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AGAAATGCCCAGAAACTCTCA AGAAGGTCTACGGTTCGAGT 42.9 50.0 57.2 57.8 668-757 bp 1.0 67.8
ndhC-trnV-UAC_p2 TCAGAAATGCCCAGAAACTCT AGAAGGTCTACGGTTCGAGT 42.9 50.0 57.2 57.8 670-759 bp 1.0 67.8
ndhC-trnV-UAC_p3 CAGAAATGCCCAGAAACTCTC AGAAGGTCTACGGTTCGAGT 47.6 50.0 56.9 57.8 669-758 bp 1.0 65.2
ndhC-trnV-UAC_p4 AGAAATGCCCAGAAACTCTCA GAAGGTCTACGGTTCGAGTC 42.9 55.0 57.2 57.5 667-756 bp 1.0 65.0
ndhC-trnV-UAC_p5 TCAGAAATGCCCAGAAACTCT GAAGGTCTACGGTTCGAGTC 42.9 55.0 57.2 57.5 669-758 bp 1.0 65.0