Hotspot

trnF-GAA-ndhJ

LSC · 51103-51382 · 280 bp

Back to Thymelaea

Pi 0.0993

MarkerSeek score 82.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 280
variable_sites: 27
pi: 0.0993
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	CATCCACGACTGAAGCGTAT	50.0	50.0	58.1	57.8	419-567 bp	1.0	83.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCACGACTGAAGCGTATCTT	50.0	50.0	58.1	57.7	416-564 bp	1.0	83.1
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	AATCATCCACGACTGAAGCG	50.0	50.0	58.1	58.4	422-570 bp	1.0	82.4
trnF-GAA-ndhJ_p4	AATGGTCGGGATAGCTCAGT	CATCCACGACTGAAGCGTAT	50.0	50.0	58.2	57.8	462-610 bp	1.0	81.9
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	CATCCACGACTGAAGCGTAT	50.0	50.0	58.2	57.8	461-609 bp	1.0	81.9