Hotspot

petN-psbM

LSC · 30297-31381 · 1085 bp

Back to Thymelaea

Pi 0.0398

MarkerSeek score 64.30

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 27
pi: 0.0398
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.62
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	AAACAGTCAGCCAAGGCAAT	45.0	45.0	58.0	58.3	780-1184 bp	1.0	83.8
petN-psbM_p2	TCTTGCTTGGGCTGCTTTAA	AACAGTCAGCCAAGGCAATT	45.0	45.0	58.0	58.3	779-1183 bp	1.0	83.8
petN-psbM_p3	AGTAAGTCTTGCTTGGGCTG	AAACAGTCAGCCAAGGCAAT	50.0	45.0	57.8	58.3	786-1190 bp	1.0	82.3
petN-psbM_p4	AGTAAGTCTTGCTTGGGCTG	AACAGTCAGCCAAGGCAATT	50.0	45.0	57.8	58.3	785-1189 bp	1.0	82.3
petN-psbM_p5	TCTTGCTTGGGCTGCTTTAA	AAAACAGTCAGCCAAGGCAA	45.0	45.0	58.0	58.5	781-1185 bp	1.0	82.0