Hotspot

psbA-trnK-UUU

LSC · 1514-1796 · 283 bp

Back to Thymelaea

Pi 0.0502

MarkerSeek score 71.40

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 283
variable_sites: 14
pi: 0.0502
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AACACACCAAACCATCCGAT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	432-433 bp	1.0	76.2
psbA-trnK-UUU_p2	ACACACCAAACCATCCGATG	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.5	58.6	431-432 bp	1.0	74.7
psbA-trnK-UUU_p3	CACACCAAACCATCCGATGT	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.5	58.6	430-431 bp	1.0	74.7
psbA-trnK-UUU_p4	AACACACCAAACCATCCGAT	ACTAGTTCCGGGTTCGAGTC	45.0	55.0	57.7	58.8	422-423 bp	1.0	74.5
psbA-trnK-UUU_p5	AACACACCAAACCATCCGAT	GACTAGTTCCGGGTTCGAGT	45.0	55.0	57.7	58.8	423-424 bp	1.0	74.5