Hotspot

trnH-GUG-psbA

LSC · 109-451 · 343 bp

Back to Thymelaea

Pi 0.0893
MarkerSeek score 75.90
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
343
variable_sites
30
pi
0.0893
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTGCCTTAATCCACTTGGCT TGAAGCTCCATCTACAAATGG 50.0 42.9 57.9 55.7 397-403 bp 1.0 59.6
trnH-GUG-psbA_p2 ACTGCCTTAATCCACTTGGC TGAAGCTCCATCTACAAATGG 50.0 42.9 58.2 55.7 398-404 bp 1.0 59.3
trnH-GUG-psbA_p3 AATCCACTTGGCTACATCCG TGAAGCTCCATCTACAAATGG 50.0 42.9 57.7 55.7 390-396 bp 1.0 58.1
trnH-GUG-psbA_p4 CTGCCTTAATCCACTTGGCT TTGAAGCTCCATCTACAAATGG 50.0 40.9 57.9 56.4 398-404 bp 1.0 57.6
trnH-GUG-psbA_p5 ACTGCCTTAATCCACTTGGC TTGAAGCTCCATCTACAAATGG 50.0 40.9 58.2 56.4 399-405 bp 1.0 57.4