Hotspot

rps16

LSC · 4963-6127 · 1165 bp

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Pi 0.00173
MarkerSeek score 60.40
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1165
variable_sites
2
pi
0.00173
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 GGTACTAGTTACCCTATCCTT TGTATCGGTTCAGCCAATGA 42.9 45.0 52.9 56.9 1272-1280 bp 1.0 40.6
rps16_p2 GGTACTAGTTACCCTATCCTT GTATCGGTTCAGCCAATGACT 42.9 47.6 52.9 58.1 1271-1279 bp 1.0 40.6
rps16_p3 GGTACTAGTTACCCTATCCTTT TGTATCGGTTCAGCCAATGA 40.9 45.0 53.8 56.9 1272-1280 bp 1.0 40.6
rps16_p4 GGTACTAGTTACCCTATCCTTT GTATCGGTTCAGCCAATGACT 40.9 47.6 53.8 58.1 1271-1279 bp 1.0 40.6
rps16_p5 GGTACTAGTTACCCTATCCTTTT TGTATCGGTTCAGCCAATGA 39.1 45.0 54.6 56.9 1272-1280 bp 1.0 40.6