Hotspot

rpoC1

LSC · 21518-24333 · 2816 bp

Back to Thunia

Pi 0.000355
MarkerSeek score 56.20
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2816
variable_sites
1
pi
0.000355
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 CCCGAATTCCCACGAGTATG ACGATCTTTGTCCCTGGAAC 55.0 50.0 58.1 57.5 2999 bp 1.0 75.2
rpoC1_p2 CCGAATTCCCACGAGTATGG ACGATCTTTGTCCCTGGAAC 55.0 50.0 58.1 57.5 2998 bp 1.0 75.2
rpoC1_p3 CGAGTATGGGCATCGCTAAT ACGATCTTTGTCCCTGGAAC 50.0 50.0 57.6 57.5 2987 bp 1.0 73.3
rpoC1_p4 CCCGAATTCCCACGAGTATG CGATCTTTGTCCCTGGAACT 55.0 50.0 58.1 57.2 2998 bp 1.0 72.8
rpoC1_p5 CCGAATTCCCACGAGTATGG CGATCTTTGTCCCTGGAACT 55.0 50.0 58.1 57.2 2997 bp 1.0 72.8