Hotspot

psbA

LSC · 125-1186 · 1062 bp

Back to Thunia

Pi 0
MarkerSeek score 29.90
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
29.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 TGGGGGATTGCTATCTTCAA GTGCTTGGGAGTCCTTGAAA 45.0 50.0 56.2 58.0 1158 bp 1.0 65.3
psbA_p2 TTGGGGGATTGCTATCTTCA GTGCTTGGGAGTCCTTGAAA 45.0 50.0 56.2 58.0 1159 bp 1.0 65.3
psbA_p3 TGGGGGATTGCTATCTTCAA AATACGTGTGCTTGGGAGTC 45.0 50.0 56.2 57.9 1165 bp 1.0 64.7
psbA_p4 TTGGGGGATTGCTATCTTCA AATACGTGTGCTTGGGAGTC 45.0 50.0 56.2 57.9 1166 bp 1.0 64.7
psbA_p5 ACAAGATATTGGGGGATTGCT GTGCTTGGGAGTCCTTGAAA 42.9 50.0 57.0 58.0 1167 bp 1.0 64.0