Hotspot

petD

LSC · 79708-81083 · 1376 bp

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Pi 0
MarkerSeek score 58.80
Primer available yes
Label petD

Feature Metadata

label_name
petD
feature_type
gene
region
LSC
length_bp
1376
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD_p1 AGGCATGTTATTTGGATACTTCT AAGGAAGATCCAGAAGGGGA 34.8 50.0 55.7 57.3 1498-1499 bp 1.0 40.1
petD_p2 AAGGCATGTTATTTGGATACTTCT AAGGAAGATCCAGAAGGGGA 33.3 50.0 56.4 57.3 1499-1500 bp 1.0 40.1
petD_p3 ACGAATCGAATAGTTGAAGT AAGGAAGATCCAGAAGGGGA 35.0 50.0 52.4 57.3 1446-1447 bp 1.0 40.1
petD_p4 AGGCATGTTATTTGGATACTTC AAGGAAGATCCAGAAGGGGA 36.4 50.0 54.2 57.3 1498-1499 bp 1.0 40.1
petD_p5 GGCATGTTATTTGGATACTTCT AAGGAAGATCCAGAAGGGGA 36.4 50.0 54.2 57.3 1497-1498 bp 1.0 40.1