Hotspot

psbB-psbT

LSC · 76739-77330 · 592 bp

Back to Thunia

Pi 0.00169
MarkerSeek score 59.60
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
592
variable_sites
1
pi
0.00169
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 TCAGAGATGTTTTTGCCGGT TGGAACTTTAGGTGGTTCTCG 45.0 47.6 57.7 57.6 786 bp 1.0 66.8
psbB-psbT_p2 TCAGAGATGTTTTTGCCGGT GGAACTTTAGGTGGTTCTCGA 45.0 47.6 57.7 57.3 785 bp 1.0 64.6
psbB-psbT_p3 GCCAGAACCTTGTTCAGAGA TGGAACTTTAGGTGGTTCTCG 50.0 47.6 57.4 57.6 799 bp 1.0 64.5
psbB-psbT_p4 GCTCAAGTGGAATTTGGAGC TGGAACTTTAGGTGGTTCTCG 50.0 47.6 57.4 57.6 748 bp 1.0 63.8
psbB-psbT_p5 GCCAGAACCTTGTTCAGAGA GGAACTTTAGGTGGTTCTCGA 50.0 47.6 57.4 57.3 798 bp 1.0 62.3