Hotspot

psbE-petL

LSC · 66795-67980 · 1186 bp

Back to Thunia

Pi 0.000843
MarkerSeek score 59.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1186
variable_sites
1
pi
0.000843
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA GCTGGGGTTATAGTTGAAGCA 50.0 47.6 57.9 58.0 1349 bp 1.0 71.6
psbE-petL_p2 CAACCCGCAATGAATAGGGA GGGTTATAGTTGAAGCAGCCA 50.0 47.6 57.9 58.0 1345 bp 1.0 71.6
psbE-petL_p3 CAACCCGCAATGAATAGGGA TGGGGTTATAGTTGAAGCAGC 50.0 47.6 57.9 58.0 1347 bp 1.0 71.6
psbE-petL_p4 ACTGACGAATAACCAACCCG GGGTTATAGTTGAAGCAGCCA 50.0 47.6 57.9 58.0 1358 bp 1.0 71.3
psbE-petL_p5 ACTGACGAATAACCAACCCG TGGGGTTATAGTTGAAGCAGC 50.0 47.6 57.9 58.0 1360 bp 1.0 71.3