Hotspot

petA-psbJ

LSC · 64964-66030 · 1067 bp

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Pi 0
MarkerSeek score 58.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1067
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCATCTGTGATTTTGGCAC TATTCCGGGTTGGGTTCATC 50.0 50.0 58.0 57.3 1178-1179 bp 1.0 74.3
petA-psbJ_p2 GGCATCTGTGATTTTGGCAC CTATTCCGGGTTGGGTTCAT 50.0 50.0 58.0 57.3 1179-1180 bp 1.0 74.2
petA-psbJ_p3 GAGTCCAAGGCCTTCTGTTC TATTCCGGGTTGGGTTCATC 55.0 50.0 58.2 57.3 1203-1204 bp 1.0 72.8
petA-psbJ_p4 GAGTCCAAGGCCTTCTGTTC CTATTCCGGGTTGGGTTCAT 55.0 50.0 58.2 57.3 1204-1205 bp 1.0 72.8
petA-psbJ_p5 GTCCAAGGCCTTCTGTTCTT TATTCCGGGTTGGGTTCATC 50.0 50.0 57.7 57.3 1201-1202 bp 1.0 72.1