Hotspot

accD-psaI

LSC · 60655-61399 · 745 bp

Back to Thunia

Pi 0.0094
MarkerSeek score 64.20
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
745
variable_sites
7
pi
0.0094
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GCTACATGGTTTCCTTCCGA AAAGAAGCCATTGCGATTGC 50.0 45.0 57.6 58.0 859-957 bp 1.0 77.4
accD-psaI_p2 AGCTACATGGTTTCCTTCCG AAAGAAGCCATTGCGATTGC 50.0 45.0 57.6 58.0 860-958 bp 1.0 77.4
accD-psaI_p3 AGGGCTTATTCGACCCAATC AAAGAAGCCATTGCGATTGC 50.0 45.0 57.4 58.0 923-1021 bp 1.0 75.5
accD-psaI_p4 GACCCAATCGTACCACGTAA AAAGAAGCCATTGCGATTGC 50.0 45.0 57.3 58.0 912-1010 bp 1.0 75.4
accD-psaI_p5 GCTACATGGTTTCCTTCCGA CGATTGCCGGAAAGACTAGG 50.0 55.0 57.6 58.4 846-944 bp 1.0 74.0