Hotspot

ndhJ-ndhK

LSC · 50824-50904 · 81 bp

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Pi 0.0123
MarkerSeek score 59.40
Primer available yes
Label ndhJ-ndhK

Feature Metadata

label_name
ndhJ-ndhK
feature_type
igs
region
LSC
length_bp
81
variable_sites
1
pi
0.0123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhJ-ndhK_p1 GGAATCCCAATCCTCGGTTT TAAGGCAGGGTTCCTTTGTG 50.0 50.0 57.6 57.7 223 bp 1.0 74.6
ndhJ-ndhK_p2 GGAATCCCAATCCTCGGTTT CAGGGTTCCTTTGTGCAGAA 50.0 50.0 57.6 58.3 218 bp 1.0 74.4
ndhJ-ndhK_p3 TGGAATCCCAATCCTCGGTT TAAGGCAGGGTTCCTTTGTG 50.0 50.0 58.7 57.7 224 bp 1.0 72.4
ndhJ-ndhK_p4 TGGAATCCCAATCCTCGGTT CAGGGTTCCTTTGTGCAGAA 50.0 50.0 58.7 58.3 219 bp 1.0 72.3
ndhJ-ndhK_p5 GGAATCCCAATCCTCGGTTT AGGGTTCCTTTGTGCAGAAT 50.0 45.0 57.6 57.3 217 bp 1.0 71.4