Hotspot

trnC-GCA-petN

LSC · 29074-29959 · 886 bp

Back to Thunia

Pi 0.00113
MarkerSeek score 59.40
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
886
variable_sites
1
pi
0.00113
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 GGTATGGCCAAGTGGTAAGG GCTCTAAAGCCCACTCCTTC 55.0 55.0 57.9 58.0 1058 bp 1.0 79.4
trnC-GCA-petN_p2 GGTATGGCCAAGTGGTAAGG AGCCCAAGCGAGACTTACTA 55.0 50.0 57.9 58.2 992 bp 1.0 78.4
trnC-GCA-petN_p3 GGTATGGCCAAGTGGTAAGG CCTTCCCCATACTACAAGCG 55.0 55.0 57.9 57.8 1043 bp 1.0 77.7
trnC-GCA-petN_p4 CCAGTTCGAATCTGGGTGTC GCTCTAAAGCCCACTCCTTC 55.0 55.0 58.3 58.0 1014 bp 1.0 77.6
trnC-GCA-petN_p5 CGGTATGGCCAAGTGGTAAG GCTCTAAAGCCCACTCCTTC 55.0 55.0 58.3 58.0 1059 bp 1.0 77.1