Hotspot

rpoB-trnC-GCA

LSC · 27573-28991 · 1419 bp

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Pi 0
MarkerSeek score 36.20
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1419
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
36.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 AATTCTTCCGTCAAGCCCTG CCTTACCACTTGGCCATACC 50.0 55.0 58.2 57.9 1546 bp 1.0 78.2
rpoB-trnC-GCA_p2 AATTCTTCCGTCAAGCCCTG CTTACCACTTGGCCATACCG 50.0 55.0 58.2 58.3 1545 bp 1.0 75.9
rpoB-trnC-GCA_p3 AAATTCTTCCGTCAAGCCCT CCTTACCACTTGGCCATACC 45.0 55.0 57.4 57.9 1547 bp 1.0 74.9
rpoB-trnC-GCA_p4 AATTCTTCCGTCAAGCCCTG TAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 57.5 1567 bp 1.0 74.4
rpoB-trnC-GCA_p5 CTTCCGTCAAGCCCTGATTA CCTTACCACTTGGCCATACC 50.0 55.0 57.3 57.9 1542 bp 1.0 74.0