Hotspot

ycf1

SSC · 122285-127807 · 5523 bp

Back to Thunbergia

Pi 0.043

MarkerSeek score 66.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5523
variable_sites: 236
pi: 0.043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTCCACCGCTTTGAATCGA	GCAGAATACCGTCACCCATT	45.0	50.0	57.9	58.0	419-428 bp	1.0	86.0
ycf1_p2	TCGATTCCACCGCTTTGAAT	GCAGAATACCGTCACCCATT	45.0	50.0	57.9	58.0	422-431 bp	1.0	86.0
ycf1_p3	TCCACCGCTTTGAATCGAAA	GCAGAATACCGTCACCCATT	45.0	50.0	58.1	58.0	417-426 bp	1.0	86.0
ycf1_p4	TTCCACCGCTTTGAATCGAA	GCAGAATACCGTCACCCATT	45.0	50.0	58.1	58.0	418-427 bp	1.0	86.0
ycf1_p5	CTTTCGAAAGAGGCCCCTTT	GCAGAATACCGTCACCCATT	50.0	50.0	58.1	58.0	282 bp	0.5	59.0