Hotspot

rpl22

LSC · 83301-83780 · 480 bp

Back to Thunbergia

Pi 0.0417

MarkerSeek score 66.90

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 480
variable_sites: 20
pi: 0.0417
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CGCCCTGCAATTTGTACTTG	CACAACCCCTTGGGGTTATC	50.0	55.0	58.0	58.2	1598-1645 bp	1.0	81.8
rpl22_p2	CGCCCTGCAATTTGTACTTG	GATTGTTCGTCGCCGTAGTA	50.0	50.0	58.0	57.8	1529-1576 bp	1.0	81.8
rpl22_p3	CGCCCTGCAATTTGTACTTG	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.0	57.8	1589-1636 bp	1.0	81.4
rpl22_p4	CGCCCTGCAATTTGTACTTG	TAATTTCCGAGGCCATGCAA	50.0	45.0	58.0	57.9	1212 bp	0.5	58.8
rpl22_p5	CGCCCTGCAATTTGTACTTG	TTAATTTCCGAGGCCATGCA	50.0	45.0	58.0	57.9	1213 bp	0.5	58.8