Hotspot

ndhF

SSC · 109323-111599 · 2277 bp

Back to Thunbergia

Pi 0.0338

MarkerSeek score 61.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2277
variable_sites: 77
pi: 0.0338
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGGCCCTCTTATCTCTTCC	AGTTGAGTTGACTTGGTGCA	55.0	45.0	57.9	57.6	2973 bp	0.5	55.8
ndhF_p2	GGGCCCTCTTATCTCTTCCT	AGTTGAGTTGACTTGGTGCA	55.0	45.0	57.9	57.6	2972 bp	0.5	55.8
ndhF_p3	TCCGAGCTGAGGTTATGGAA	AGTTGAGTTGACTTGGTGCA	50.0	45.0	58.1	57.6	2950 bp	0.5	55.5
ndhF_p4	CCGAGCTGAGGTTATGGAAG	AGTTGAGTTGACTTGGTGCA	55.0	45.0	57.8	57.6	2949 bp	0.5	54.8
ndhF_p5	CGTAGGGCCCTCTTATCTCT	AGTTGAGTTGACTTGGTGCA	55.0	45.0	57.7	57.6	2976 bp	0.5	54.3