Hotspot

ccsA-ndhD

SSC · 114079-114312 · 234 bp

Back to Thunbergia

Pi 0.0694

MarkerSeek score 72.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 234
variable_sites: 15
pi: 0.0694
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GAGCTGGGACCCAAAAGAAA	GTCCTCCCTTGCATTACCAG	50.0	55.0	58.0	58.0	800 bp	0.5	59.6
ccsA-ndhD_p2	CTGTGCCACTTCAGGTCTTT	GTCCTCCCTTGCATTACCAG	50.0	55.0	58.0	58.0	1223 bp	0.5	59.4
ccsA-ndhD_p3	GAGCTGGGACCCAAAAGAAA	CACCGATATGGGACTGAACG	50.0	55.0	58.0	58.1	980 bp	0.5	58.8
ccsA-ndhD_p4	CTAACGAAGCATGGGGATCC	GTCCTCCCTTGCATTACCAG	55.0	55.0	58.1	58.0	825 bp	0.5	58.7
ccsA-ndhD_p5	GAGCTGGGACCCAAAAGAAA	GATATGGGACTGAACGGAGC	50.0	55.0	58.0	57.8	976 bp	0.5	58.7