Hotspot

ndhF-rpl32

SSC · 111600-112103 · 504 bp

Back to Thunbergia

Pi 0.0586

MarkerSeek score 73.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 504
variable_sites: 29
pi: 0.0586
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CAGGAAGTCGTGTGAACCAA	CGGTAAGGCAAAGAGACGAA	50.0	50.0	58.1	57.9	1369-1371 bp	1.0	83.8
ndhF-rpl32_p2	CAGGAAGTCGTGTGAACCAA	TTCGGTAAGGCAAAGAGACG	50.0	50.0	58.1	57.9	1371-1373 bp	1.0	83.8
ndhF-rpl32_p3	CAGGAAGTCGTGTGAACCAA	AAAAGGCTTTTAACGCTGCC	50.0	45.0	58.1	57.9	1105-1112 bp	1.0	82.7
ndhF-rpl32_p4	CCCACAGACGACGAAGATTT	CGGTAAGGCAAAGAGACGAA	50.0	50.0	57.9	57.9	981 bp	0.5	58.8
ndhF-rpl32_p5	CCCACAGACGACGAAGATTT	TTCGGTAAGGCAAAGAGACG	50.0	50.0	57.9	57.9	983 bp	0.5	58.8