Hotspot

rpl22-rps19

LSC · 83781-83988 · 208 bp

Back to Thunbergia

Pi 0.12

MarkerSeek score 72.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 208
variable_sites: 25
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGGGGTTTGAATTTCTTCGC	AAAAGTTTGGGTAGAGCCGG	50.0	50.0	58.0	58.1	1049-1096 bp	1.0	83.2
rpl22-rps19_p2	CGGGGTTTGAATTTCTTCGC	ATTACCTTCTGGGGAGGTCC	50.0	55.0	58.0	58.1	1131-1178 bp	1.0	82.8
rpl22-rps19_p3	CGGGGTTTGAATTTCTTCGC	TGGGGAGGTCCGTTTGATAT	50.0	50.0	58.0	58.1	1122-1169 bp	1.0	82.8
rpl22-rps19_p4	CGGGGTTTGAATTTCTTCGC	GCGAACCAGAAAAGTTTGGG	50.0	50.0	58.0	57.9	1058-1105 bp	1.0	82.8
rpl22-rps19_p5	CGGGGTTTGAATTTCTTCGC	GGCGAACCAGAAAAGTTTGG	50.0	50.0	58.0	57.9	1059-1106 bp	1.0	82.8