Hotspot

psaJ-rpl33

LSC · 67323-67817 · 495 bp

Back to Thunbergia

Pi 0.045

MarkerSeek score 72.90

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 22
pi: 0.045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTCCCAGATGCGTTGACA	TTACTCGCGCATCTTTCCTC	45.0	50.0	58.0	58.1	564-573 bp	1.0	83.1
psaJ-rpl33_p2	GGTTTTTCCCAGATGCGTTG	TTACTCGCGCATCTTTCCTC	50.0	50.0	57.9	58.1	567-576 bp	1.0	82.8
psaJ-rpl33_p3	ATGCGTTGACATTCCCCTTT	TTACTCGCGCATCTTTCCTC	45.0	50.0	58.1	58.1	555-564 bp	1.0	82.8
psaJ-rpl33_p4	TTTTCCCAGATGCGTTGACA	CAAAATCACCGTTACTCGCG	45.0	50.0	58.0	57.9	575-584 bp	1.0	82.5
psaJ-rpl33_p5	GGTTTTTCCCAGATGCGTTG	CAAAATCACCGTTACTCGCG	50.0	50.0	57.9	57.9	578-587 bp	1.0	82.2