Hotspot

rbcL-accD

LSC · 56901-57601 · 701 bp

Back to Thunbergia

Pi 0.0447

MarkerSeek score 71.90

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 31
pi: 0.0447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	ACTACAGTTCGGTGGAGGAA	ACTCTTCTCGGGGGTTTGTA	50.0	50.0	58.0	58.0	1548 bp	0.5	59.8
rbcL-accD_p2	ACTACAGTTCGGTGGAGGAA	TACTCTTCTCGGGGGTTTGT	50.0	50.0	58.0	58.0	1549 bp	0.5	59.8
rbcL-accD_p3	TTGAATGCTACTGCGGGTAC	ACTCTTCTCGGGGGTTTGTA	50.0	50.0	58.0	58.0	2027 bp	0.5	59.7
rbcL-accD_p4	TTGAATGCTACTGCGGGTAC	TACTCTTCTCGGGGGTTTGT	50.0	50.0	58.0	58.0	2028 bp	0.5	59.7
rbcL-accD_p5	TCATGGTATGCACTTCCGTG	ACTCTTCTCGGGGGTTTGTA	50.0	50.0	58.0	58.0	1827 bp	0.5	59.7